For use on the Rotor-Gene Q. Rotor-Gene Q Software 2.3 is compatible with both Windows XP and Windows 7 operating systems. If you would like to upgrade the operating system for your QIAGEN computer (HP ProBook 6550b or HP ProBook 6570b) to Windows 7, please contact QIAGEN Technical Services for your. Created by Bio Molecular Systems. The original team behind Rotor-Gene 6000™. We’re able to optimise quality and ensure rapid product development.

HRM Introduction High Resolution Melting ( HRM) is a novel, homogeneous, close-tube, post-PCR method, enabling genomic researchers to analyze genetic variations (SNPs, mutations, methylations) in PCR amplicons. It goes beyond the power of classical melting curve analysis by allowing to study the thermal denaturation of a double-stranded DNA in much more detail and with much higher information yield than ever before. HRM characterizes nucleic acid samples based on their disassociation (melting) behavior. Samples can be discriminated according to their sequence, length, GC content or strand complementarity. Even single base changes such as SNPs (single nucleotide polymorphisms) can be readily identified. HRM Applications The introduction of HRM has renewed interest in the utility of DNA melting for a wide range of uses, including: • Mutation discovery (gene scanning) • Screening for loss of heterozygosity • DNA fingerprinting • SNP genotyping • Characterization of haplotype blocks • DNA methylation analysis • DNA mapping • Species identification • Somatic acquired mutation ratios • HLA compatibility typing • Association (case/control) studies • Allelic prevalence in a population • Identification of candidate predisposition genes. In molecular biology High Resolution Melt or HRM analysis as it will be referred to herein is a hugely powerful technique for the detection of mutations, polymorphisms and epigenetic differences in double stranded DNA samples.

It has advantages over other genotyping technologies. Stronghold Crusader 2 Free Download Chip. Namely: • It is massively cost effective vs. Other genotyping technologies such as sequencing and Taqman SNP typing. This makes it ideal for large scale genotyping projects.

• It is fast and powerful thus able to accurately genotype huge numbers of samples in rapid time. • It is simple.

With a good quality HRM assay powerful genotyping can be performed by non-geneticists in any laboratory with access to an HRM capable real-time PCR machine. HRM Instrumentation For several years, various researchers and instrument makers have independently investigated the utility of high-resolution DNA dissociation analysis. For example, the team at Idaho Technology has done an admirable job of vigorously promoting their research through traditional journal publications.